An electrochemical oxidation of acetaminophen (ACOP) has been successfully performed by using glassy carbon electrode covered with 4-hydroxyquinoline-3-carboxylic acid (4HQ3CA) to reinforce electrode’s feature. To characterize the modified electrode (4HQ3CA/GC), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and Fourier transform infrared spectroscopy (FT-IR) techniques were used. The finding optimum conditions (supporting electrolyte, pH) and the electrochemical determination studies were performed with differential pulse voltammetry (DPV). A wide concentration range from 0.0025 μM to 141 μM with a limit of detection (LOD) of 5.98×10-10 M (3s/m) was obtained. This prepared sensor was carried out for the determination of ACOP in pharmaceutical sample.