Assessment of anticholinesterase and antioxidant properties of the extracts and (+)-catechin obtained from Arceuthobium oxycedri (D.C.) M. Bieb (dwarf mistletoe)

Orhan İ., Akkol E. K., Suntar İ., Yesilada E.

South African Journal of Botany, cilt.120, ss.309-312, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 120
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.sajb.2018.09.023
  • Dergi Adı: South African Journal of Botany
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.309-312
  • Anahtar Kelimeler: Arceuthobium, Santalaceae, Cholinesterase inhibition, Antioxidant activity, Catechin, FOLK MEDICINE, INHIBITORS, FLAVONOIDS
  • Gazi Üniversitesi Adresli: Evet

Özet

© 2018 South African Association of BotanistsThe hexane, chloroform, ethyl acetate, water, and n-butanol extracts of Arcethobium oxycedri (DC.) M.Bieb. (Santalaceae) were evaluated for their inhibitory effects against acetyl- (AChE) and butyrylcholinesterase (BChE), related to Alzheimer's disease at 200 μg/mL using microplate assays. Since oxidative stress contributes much to neurodegeneration, antioxidative effect of the extracts was determined in metal-chelating and ferric-reducing antioxidant power (FRAP) test systems as well as antiradical effect against 2,2-diphenyl-1-picrylhydrazyl (DPPH). Total phenol and flavonoid contents of the extracts were calculated spectrophotometrically. Our findings indicated that the EtOAc extract, the richest one in total phenol and flavonoid amount (455.22 ± 4.35 and 194.45 mg/g extract, respectively) displayed the highest cholinesterase inhibition (45.37 ± 1.23% for AChE and 94.74 ± 2.43% for BChE at 200 μg/mL). Fractionation of the EtOAc led to isolation of (+)-catechin as the major compound. Catechin exerted remarkable antioxidant effect, whereas it did not cause cholinesterase inhibition. Therefore, the EtOAc extract deserves further investigation for its high BChE inhibition.