Akademik Veri Yönetim Sistemi
Turkish Journal of Botany, cilt.43, sa.5, ss.585-596, 2019 (SCI-Expanded)
The genus Adonis L. is a member of Ranunculaceae and consists of perennial and annual herbaceous plants included in thetribe Adonideae under the subfamily Ranunculoideae. Botanically, Ranunculaceae comprises vital medicinal plants. Molecular markersare one of the most effective tools for exploring genetic variation that can enhance breeding efficiency. To identify the genetic diversityof 62 Adonis ecotypes collected from different regions in Turkey, the interprimer binding site (iPBS) retrotransposon system was used.Of the 83 iPBS primers used, 10 provided sufficient polymorphic data, generating a total of 204 alleles. The number of iPBS bandsper individual was 3.29, and the number of alleles per polymorphic locus ranged from 8 to 35, with an average of 20.30. The averagepolymorphism percentage was 99.50%, and polymorphic information content ranged from 0.16 to 0.39. The highest average numberof alleles, Nei’s genetic diversity (h), and Shannon’s information index (I) were obtained from A. volgensis species (1.64, 0.39, and 0.58,respectively), whereas the lowest values (1.41, 0.29, and 0.46, respectively) were found in A. flammea species. The analysis of molecularvariance revealed significant variance within the population (71%), whereas no significant genetic variation was observed among thedifferent species (29%). Cluster analysis according to unweighted pair-group mean average (UPGMA) divided 62 Adonis ecotypes intofour major clusters. According to the principal coordinate analysis, the first three principal coordinates accounted for 81.51% of totalvariation. Genetic structure analysis of the studied germplasm using the Bayesian method revealed four subpopulations with an averageof 0.2634 for expected heterozygosity and 0.2154 for population differentiation measurements. The results of this study suggested thatiPBS markers could be used in the identification of genetic diversity among the Adonis species.