Expression of ALS1, HWP1 and SAP4 genes in Candida albicans strains isolated from women with vaginitis

Nas T., Kalkanci A., Fidan I., Hizel K., Bolat S., Yolbakan S., ...Daha Fazla

FOLIA MICROBIOLOGICA, cilt.53, sa.2, ss.179-183, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 53 Sayı: 2
  • Basım Tarihi: 2008
  • Dergi Adı: FOLIA MICROBIOLOGICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.179-183
  • Gazi Üniversitesi Adresli: Evet

Özet

Total mRNA of Candida strains (isolated from whole vaginal swabs) was investigated and the in vivo expression of C albicans secreted aspartyl proteinase (SAP4), aglutinin-like sequence (ALS1) and hyphal wall protein (HWP1) genes was determined. A spectrum of gene expression profiles of strains isolated from vulvovaginal candidiasis (VVC) cases consisting of 10 pregnant, 4 postmenopausal, and 15 reproductive age (12 primary and 3 recurrent) women with different estrogen level. Expression of SAP4, ALS1 and HWP1 genes was evaluated by reverse-transcriptase polymerase chain reaction using specific primer sets. The expression of ALS1, HWP1, and SAP4 was detected as 69, 62, and 38 %, respectively, in all cases. In pregnant, postmenopausal, and reproductive age women with VVC, the expression of ALS1 was observed as 70, 75, 67 %, and HWP 60, 25, 73 %, respectively. Expression of SAP4 was found in pregnant, postmenopausal, and reproductive age women as 40, 50, and 33 %, respectively. Expression of the adhesion genes in VVC does not correlate with estrogen level of the patients.