Effects of co-administered melatonin, fructose and bisphenol A (BPA) on rat epididymis and sperm characteristics

Akarca-Dizakar S. Ö., Erdogan D., Peker T. V., Akcay N. C., Turkoglu I., Esmekaya M. A., ...Daha Fazla

BIOTECHNIC & HISTOCHEMISTRY, cilt.95, sa.1, ss.18-26, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 95 Sayı: 1
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1080/10520295.2019.1627418
  • Dergi Adı: BIOTECHNIC & HISTOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.18-26
  • Anahtar Kelimeler: Bisphenol A, epididymis, fructose, melatonin, sperm, METABOLIC SYNDROME, TIGHT JUNCTIONS, IN-VIVO, EXPOSURE, QUALITY, XENOESTROGENS, DISRUPTION, EXPRESSION, EPITHELIUM, BARRIER
  • Gazi Üniversitesi Adresli: Evet

Özet

Consumption of fructose-rich food and exposure to endocrine disrupting chemicals continue to increase. High fructose consumption is associated with increased incidence of dyslipidemia, hypertension, hyperuricemia and insulin resistance. Bisphenol A (BPA) is an environmental contaminant that exhibits estrogen-like activity; it impairs reproductive organs, sperm production, spermatogenesis and fertility. We investigated the possible ameliorative effects of melatonin on rat epididymis and sperm characteristics following exposure to fructose and BPA. We used 42 adult male Sprague-Dawley rats divided into seven groups. Group 1, control group, was treated with 25 mg/kg sesame oil + 25 mg/kg 0.1% ethanol. Group 2 was treated with 10% aqueous fructose. Group 3 was treated with 25 mg/kg BPA. Group 4 was treated with 10% fructose and 25 mg/kg BPA. Group 5 was treated with 10% fructose and 20 mg/kg melatonin. Group 6 was treated with 25 mg/kg BPA and 20 mg/kg melatonin. Group 7 was treated with 10% fructose, 25 mg/kg BPA and 20 mg/kg melatonin. After 60 days, epididymal tissue was removed and analyzed using histochemistry and immunohistochemistry. Sperm were counted, and sperm motility and viability were investigated. Administration of BPA caused significant damage to both epididymal tissue and sperm quality; melatonin reduced the damage, but did not prevent it completely.