Gingival tissue and crevicular fluid co-operation in adult periodontitis

Beklen A., Tuter G., Sorsa T., Hanemaaijer R., Virtanen I., Tervahartiala T., ...More

JOURNAL OF DENTAL RESEARCH, vol.85, no.1, pp.59-63, 2006 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 85 Issue: 1
  • Publication Date: 2006
  • Doi Number: 10.1177/154405910608500110
  • Journal Name: JOURNAL OF DENTAL RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.59-63
  • Keywords: stromelysin, collagenase, gelatinase, periodontitis, MATRIX METALLOPROTEINASE-3 STROMELYSIN, NECROSIS-FACTOR-ALPHA, PORPHYROMONAS-GINGIVALIS, TREPONEMA-DENTICOLA, NEUTROPHIL ELASTASE, CATHEPSIN-G, ACTIVATION, CELLS, GELATINASE, INHIBITOR
  • Gazi University Affiliated: Yes

Abstract

Activated matrix metalloproteinase-3 (MMP- 3) can contribute to periodontal ligament destruction in adult periodontitis. Since MMP- 3 has been reported to activate proMMP-8 and -9, it was speculated that gingival tissue fibroblast- derived MMP-3 might, in periodontitis, be responsible for activation of gingival crevicular fluid (GCF) neutrophil- derived proMMP-8 and -9. Immunohistochemistry disclosed MMP-3 in gingival fibroblasts in periodontitis. Cultured gingival fibroblasts released only pro-MMP-3 when stimulated with tumor necrosis factor-alpha. However, Western blot revealed partially activated MMP-3, MMP-8, and MMP-9 in periodontitis GCF. Active MMP-8 (p < 0.05) and MMP-9 (p < 0.05) correlated with the presence of active MMP-3. It seems that resident gingival fibroblasts produce pro-MMP-3 in GCF, where it becomes activated, probably by cathepsin G or elastase released by neutrophils. Active MMP-3 then activates neutrophil-derived pro- MMP-8 and -9. Different tissue compartments/cells exert co-operative actions in mutual local MMP activation cascades.