The novel approach towards estimation of frost tolerance in barley

HALİLOĞLU K., TOSUN M., Yildirim T., AYDIN M.

Biotechnology and Biotechnological Equipment, cilt.23, sa.1, ss.1131-1135, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23 Sayı: 1
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1080/13102818.2009.10817627
  • Dergi Adı: Biotechnology and Biotechnological Equipment
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1131-1135
  • Anahtar Kelimeler: Artificial freezing test, Cold-induced genes, PCR-based frost resistance test
  • Gazi Üniversitesi Adresli: Evet

Özet

We developed a convenient, efficient and reliable PCR-based technique to distinguish the frost tolerant and sensitive genotypes in barley. Based on artificial freeze test, significant differences among genotypes were found. In these plants, frost tolerance, expressed as LT50, was strongly correlated with the existence of cold-induced genes. Comparison of LT50 values and the PCR-detected pattern of cold-induced genes showed that Hudson, Dictoo and Krusevac occupied three first positions in terms of a high frost tolerance. After 35 and 49 days of cold acclimation, the Nortstar was found to be the most tolerant wheat genotype (LT50: -16°C). Low correlation between LT50 and existence of cold-induced genes was found for rye and wheat genotypes. Diploid forms of rye plants were found to be more frost tolerant than tetraploid forms. © 2009 Taylor and Francis Group, LLC.