The effect of L-NAME administrations after oral mucosal incision on wound NO level in rabbit.


Coskun Ş., Karatas F., Acarturk F., Olmus H., Selvi M., Erbas D.

Molecular and cellular biochemistry, vol.278, no.1-2, pp.65-9, 2005 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 278 Issue: 1-2
  • Publication Date: 2005
  • Doi Number: 10.1007/s11010-005-6628-6
  • Journal Name: Molecular and cellular biochemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.65-9
  • Keywords: implant, lipid peroxidation, L-NAME, nitric oxide, oral mucosa, titanium, NITRIC-OXIDE, SKIN
  • Gazi University Affiliated: Yes

Abstract

The aim of the current study was to comparatively investigate the effect of inhibition of nitric oxide (NO) production by N-nitro-L-arginine methyl ester (L-NAME), an isoform non-specific inhibitor of nitric oxide synthase (NOS), after oral mucosal incision on wound tissue NO levels. A standard incision was applied to the oral mucosa of rabbits. After oral mucosal incision, rabbits were divided into five groups as follows: (1) Untreated incisional group (control); (2) Titanium (Ti) implanted group; (3) Ti + Polyethylene glycol (PEG) 4000 implanted group; (4) Ti + PEG 4000 + L-NAME (2 x 10(-4) M) implanted group and (5) i.p. L-NAME administrated group (10 mg/kg). At 5 days after oral incision operations, wound tissue strips and plasma were obtained from rabbits. Oral wound tissue and plasma nitric oxide, plasma thiobarbituric acid reactive substances (TBARS) and total sulfhydryl group (RSH) levels were investigated. Plasma TBARS and NOx levels decreased after i.p. L-NAME administration. Total RSH group levels were not changed in all groups (p > 0.05). This means that L-NAME inhibits the deteriorating effects of free radicals without affecting healing. L-NAME in PEG and titanium also has no effect on tissue and plasma NOx levels. These findings indicate that NO generation will not be affected both Ti and local nitric oxide synthase (NOS) inhibitor.