New supports for use of Glucose Oxidase enzyme as biocatalysis


Creative Commons License

Sarı N., Bağcacıer Z. N., Kurnaz Yetim N., Hasanoğlu Özkan E., Nartop D.

W. A. Science, Engineering and Technology, cilt.78, ss.949-953, 2013 (Hakemli Dergi)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 78
  • Basım Tarihi: 2013
  • Dergi Adı: W. A. Science, Engineering and Technology
  • Derginin Tarandığı İndeksler: Other Indexes
  • Sayfa Sayıları: ss.949-953
  • Gazi Üniversitesi Adresli: Evet

Özet

Nanoparticles modified polystyrenes with some Furfural derivatives were synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on there. The characteristics of the immobilized glucose oxidase (APS-FurH)-GOx, (APS-FurCH3)-GOx, (APS-FurNO2)-GOx enzyme showed except one of them. The insertion of stabile (SchFur-NO2) spacers between polystyrene backbone and the immobilized GOx, (APS-Fur-SchNO2GOx), increases the enzymes’ activity and improves their affinity towards the substrate even at pH = 4, enzyme showed except one of them. The insertion of stabile (SchFur-NO2) spacers between polystyrene backbone and the immobilized GOx, (APS-Fur-SchNO2GOx), increases the enzymes’ activity and improves their affinity towards the substrate even at pH = 4.