Detection of p53 deletions using fish technique in the HL-60, Daudi, Raji and K-562 cell lines

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Konac E., Ergün M. A., Sahin F., Ekmekci A.

Experimental Oncology, vol.25, pp.33-35, 2003 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 25
  • Publication Date: 2003
  • Journal Name: Experimental Oncology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.33-35
  • Gazi University Affiliated: Yes

Abstract

The work is aimed on the analysis of chromosome 17 centromere and p53 gene-specific signals within interphase and metaphase, determination of p53 deletion ratios and the detection of the cell-cycle progress. The loss of heterozygosity (LOH) has been investigated using FISH method in 4 cell lines (HL-60, Daudi, Raji and K-562) and peripheral blood samples from 3 Burkitt's lymphoma patients and 2 healthy donors. It is concluded that FISH method may be used in aneuploidy as well as in detection of p53 deletions and mutations.