Effects of fly ash and boric acid on Y2O3-stabilized tetragonal ZrO2 dispersed with MgAl2O4: An experimental study on rat subcutaneous tissue


ERGÜN G., GÜRÜ M., EĞİLMEZ F., ÇEKİÇ NAGAŞ I., Yilmaz D.

ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER, vol.199, pp.23-29, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 199
  • Publication Date: 2015
  • Doi Number: 10.1016/j.aanat.2014.02.003
  • Journal Name: ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.23-29
  • Keywords: Boric acid, Fly ash, Inflammatory reaction, Tissue response, Zirconia, CONNECTIVE-TISSUE, MAST-CELLS, ZIRCONIA, BIOCOMPATIBILITY, EOSINOPHIL, IMPLANTS, MICE
  • Gazi University Affiliated: Yes

Abstract

The aim of this study was to evaluate the subcutaneous tissue reaction around zirconia-based materials. Forty-eight male Wistar Albino rats were used in this study. Disk-shaped (1 mm height and 5 mm diameter) samples composed of 67% spinel (MgAl2O4), 27% tetragonal zirconia polyaystal, 4% (m/m) fly ash and 2% (m/m) boric acid were inserted into dorsal muscles of rats. After 1, 4, 8 and 16 weeks, the animals were sacrificed and zirconia materials were removed with the surrounding tissue. Tissue sections were made with a microtome and then stained with hematoxylin and eosin. Sections were evaluated for the intensity of inflammation. Additionally, the somatic and visceral lymph nodes were evaluated. Data were submitted to one-way analysis of variance (ANOVA) and Tukey HSD tests at a significant level of p < 0.05. There were statistically significant differences between mean inflammatory scores in different experimental periods (p < 0.05). In addition, the inflammatory reaction decreased over time. The tested materials had no damaging effect on the rat lymph nodes and did not have a toxic action on the internal organs. Therefore, zirconia polycrystal tested in the present study may offer a promising treatment alternative after further mechanical and biological studies are performed. (C) 2014 Elsevier GmbH. All rights reserved.