Multiwalled Carbon Nanotube-Chitosan Scaffold: Cytotoxic, Apoptoti and Necrotic Effects on Chondrocyte Cell Lines

İLBASMIŞ TAMER S., ÇİFTÇİ H., TÜRK M., Degim T., TAMER U.

CURRENT PHARMACEUTICAL BIOTECHNOLOGY, vol.18, no.4, pp.327-335, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 4
  • Publication Date: 2017
  • Doi Number: 10.2174/1389201018666170127105555
  • Journal Name: CURRENT PHARMACEUTICAL BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.327-335
  • Keywords: Carbon nanotubes, chitosan, scaffold, WST-1 assay, chondrocyte cell lines, nanocomposite., REGENERATIVE MEDICINE, RAMAN-SPECTROSCOPY, BONE, HYDROXYAPATITE, NANOPARTICLES, BIOCOMPATIBILITY, NANOCOMPOSITES, COMPOSITE, CARTILAGE, COLLAGEN
  • Gazi University Affiliated: Yes

Abstract

Background: Carbon nanotubes (CNTs) have been considered highly successful and proficient in terms of their mechanical, thermal and electrical functionalization and biocompatibility. In regards to their significant extent in bone regeneration, it has been determined that CNTs hold the capability to endure clinical applications through bone tissue engineering and orthopedic procedures. In the present study, we report on a composite preparation, involving the use of CNT-chitosan as scaffold for bone repair and regeneration. Through the use of water-soluble tetrazolium salt (WST-1) and double staining methods, the cytotoxic, necrotic, and apoptotic effects of chitosan-multiwalled carbon nanotube nanocomposites on the chondrocyte ATTC cell line have been exhibited.