International Conference On Nanobiotechnology and Pharmacy, İstanbul, Turkey, 9 - 10 July 2024, pp.23
The Olea europaea L., a member of the
Oleaceae family, has long been utilized in traditional medicine across
Mediterranean regions [1]. Olive leaf and extracts are popular in complementary
and alternative medicine for their potential to reduce blood pressure in
animals [2] and to act as an antipyretic and diuretic for malaria in humans
[3-5].
The
aim of this study is to determine diuretic activity and quantity of rosmarinic
acid in leaf extracts of Olea europaea L. Ayvalik, Olea europaea
L. Gemlik, and a mixture of two taxa’s leaves (50:50, w/w) in Turkey. All Olea europaea L. taxa
were extracted from their leaves using the infusion method with hot water for
two hours. According to results of UPLC-UV
analysis, the infusion extraction of a mixture of leaves with the highest
quantity of rosmarinic acid. In the in-vitro enzymatic diuretic analysis,
the infusion extraction of a mixture of leaves showed the highest inhibition of
carbonic anhydrase enzyme with 9.39 µg/mL (equivalent to acetazolamide) (Table
1).
Table
1. Results of the diuretic activity experiments
Olea europeae
Species |
Concentration
(equivalent acetazolamide) (µg/mL) |
Inhibition with
dilution factor |
Ayvalık |
1.89 |
59.38 |
Gemlik |
1.94 |
67.59 |
Mixture of species |
9.39 |
317.23 |
In light of these results, it was noted that the infusion extraction of a
mixture of leaves exhibited the most significant diuretic activity, which effect
may be associated with the level of rosmarinic acid and inhibition of carbonic
anhydrase.