Anti-Acetylcholinesterase and Antioxidant Appraisal of the Bulb Extracts of Five Sternbergia Species

ERDOĞAN ORHAN İ., Yilmaz B. S., ALTUN M. L., Saltan G., Sener B.

RECORDS OF NATURAL PRODUCTS, cilt.5, sa.3, ss.193-201, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 5 Sayı: 3
  • Basım Tarihi: 2011
  • Dergi Adı: RECORDS OF NATURAL PRODUCTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.193-201
  • Anahtar Kelimeler: Sternbergia, Amaryllidaceae, lycorine, acetylcholinesterase, antioxidant, ALKALOIDS, LYCORINE, AMARYLLIDACEAE
  • Gazi Üniversitesi Adresli: Evet

Özet

In the current study, we examined anti-acetylcholinesterase (AChE) and antioxidant activities of the ethyl acetate, methanol, and water extracts from the bulbs of Turkish Sternbergia Waldst. & Kit. (Amaryllidaceae) species; S. candida, S. clusiana, S. fisheriana, S. lutea subsp. lutea, and S. lutea subsp. sicula. Anti-AChE activity was tested by spectrophotometric method of Ellman using ELISA microplate reader at 50, 100, and 200 mu g mL(-1) concentrations. Antioxidant activity of the extracts was evaluated by DPPH radical scavenging activity, ferrous ion-chelating capacity, ferric-reducing antioxidant power, and beta-carotene bleaching assays at 500, 1000, and 2000 mu g mL(-1). Total phenol and flavonoid contents of the extracts were determined via Folin-Ciocalteau's and AlCl(3) reagents, respectively. The ethyl acetate extract of S. fischeriana was the most active in anti-AChE assay (90.94% and 98.02% of inhibitions at 100 and 200 mu g mL(-1) concentrations, respectively. Antioxidant activity of the extracts was found to be not significant.