Protective effect of adrenomedullin on contrast induced nephropathy in rats


Inal S., Koc E., Ulusal-Okyay G., PAŞAOĞLU Ö. T., IŞIK GÖNÜL İ., Oz-Oyar E., ...Daha Fazla

NEFROLOGIA, cilt.34, sa.6, ss.724-731, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 6
  • Basım Tarihi: 2014
  • Doi Numarası: 10.3265/nefrologia.pre2014.sep.12405
  • Dergi Adı: NEFROLOGIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.724-731
  • Anahtar Kelimeler: Adrenomedullin, Contrast-induced nephropathy, Radiocontrast media, Rat model, ACUTE-RENAL-FAILURE, MACROPHAGE CELL-LINE, BINDING PROTEIN-1, DOWN-REGULATION, NITRIC-OXIDE, RISK-FACTORS, INJURY, VASOCONSTRICTION, PATHOGENESIS, PREVENTION
  • Gazi Üniversitesi Adresli: Evet

Özet

Background and aims: Contrast-induced nephropathy (CIN) has a growing incidence in which renal vasoconstriction and medullary hypoxia are important mechanisms. Therapeutic approaches are very restricted and there is a considerable interest in advancing preventive strategies. Adrenomedullin is a relatively novel peptide having antioxidant, vasoactive and vasodilatory properties. We aimed to investigate whether adrenomedullin might have a preventive role against the development of experimental CIN. Methods: Wistar albino rats (n=24) were allocated randomly into four equal groups of 6 each; Control (C), Adrenomedullin (A), Contrast Media (CM) and Adrenomedullin plus Contrast Media (ACM). All rats were deprived of water from day 1 to day 4 during 72 hours. Then, intravenous administrations of chemicals were performed. Adrenomedullin was given at dose of 12 mu g/kg to groups A and ACM. A single dose of high-osmolar contrast media; diatrizoate (Urografin 76%, Schering AG, Germany) was injected to groups CM and ACM at dose of 10mL/kg. On day 1 and 6 blood samples were drawn for renal function tests and inflammatory markers including TNF-alpha IL-1 beta, IL-6 and IL-18. After sacrification, kidney histologies were examined with hematoxylineosin staining. Results: Compared to CM group, serum cystatin-C levels on 6th day were found significantly lower in ACM group (p<0.05). Additionally, daily protein excretion rates, absolute changes in daily urine output and creatinine clearance values were significantly lower in ACM group than those in CM group (p<0.05). In histopathological evaluation, regarding the degree of tubular damage and medullary congestion scores, ACM group had slightly better scores compared to CM group; however the differences did not reach significance as shown in inflammatory markers. Conclusion: This study demonstrated a beneficial impact of adrenomedullin on deteriorated renal function tests in an experimental CIN model. Adrenomedullin might be a candidate agent for prophylaxis of CIN. However, further studies are needed to shed more light on this issue.