Anti-lipoxygenase Evaluation of Different Achillea sp. Extracts

Subaşı B., GÜNBATAN T., GÜRBÜZ İ., İNAL E., KARTAL M., Sucu M., ...More

Chemistry and Biodiversity, 2025 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2025
  • Doi Number: 10.1002/cbdv.202500466
  • Journal Name: Chemistry and Biodiversity
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Keywords: Achillea, anti-inflammatory activity, hyperoside, lipoxygenase, vanillic acid
  • Gazi University Affiliated: Yes

Abstract

In this present work, in vitro lipoxygenase (LOX) inhibitory activities of methanol extracts and different fractions of six Achillea species [A. biebersteinii, A. millefolium, A. cappadocica, A. wilhelmsii, A. setacea, and A. nobilis subsp. neilreichii] were evaluated. A microplate-based spectrophotometric method was utilized for the LOX assay. The phenolic composition was analyzed using the liquid chromatography-high resolution mass spectrometer (LC-HRMS). As a result, the A. biebersteinii (Malatya locality) ethyl acetate fraction showed the highest lipoxygenase inhibitory activity (72.9% inhibition), followed by the A. cappadocica ethyl acetate fractions with 66.9% and 60.4% inhibition, respectively. The phytochemical LC-HRMS analyses revealed that the active constituents of the studied fractions were vanillic acid, hyperoside, p-coumaric acid, quercetin, and chrysoeriol. The findings showed the active bioassay-directed fractions, as well compounds to the best of our knowledge for the first time, suggesting further future biological, pharmacological, and toxicological evaluations both in vitro and in vivo.