Effects of Bacillus thuringiensis kurstaki on Malpighian tubule cells of Thaumetopoea pityocampa (Lepidoptera : Thaumetopoeidae) larvae

Ogutcu A., Suludere Z., Uzunhisarcikli M., KALENDER Y.

FOLIA BIOLOGICA-KRAKOW, vol.53, pp.7-11, 2005 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 53
  • Publication Date: 2005
  • Doi Number: 10.3409/1734916054663447
  • Journal Name: FOLIA BIOLOGICA-KRAKOW
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.7-11
  • Keywords: Malpighian tubules, Bacillus thuringiensis kurstaki, Thaumetopoea pityocampa, ultrastructure, transmission electron microscope, DELTA-ENDOTOXIN, CATERPILLAR, MIDGUT, ISRAELENSIS, TOXINS
  • Gazi University Affiliated: Yes

Abstract

In this study effects of Bacillus thuringiensis kurstaki (Btk) on Malpighian tubule cells of Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae) larvae was investigated by electron microscopy. 3 mg/l Btk was given with food. After Btk administration, the Malpighian tubule cells were investigated and compared with a control group. 3 and 6 hrs after Btk administration swelling in Malpighian tubule cells was observed. Swelling of mitochondria and separation of their cristae was seen after 12 hrs. After 24 hrs dissolution of the basal cytoplasm, swelling and vacuolization of all mitochondria, partial dissolution of the nucleoplasm, and swelling and separation of microvilli was documented. A membrane-body in the nucleus was seen after 48 hrs. The nucleoplasm was completely dissolved after 72 hrs and after 96 hrs large vacuoles appeared in the cytoplasm and shortening of microvilli was observed.