Atıf İçin Kopyala
Donmez S., Arslan F., Sarı N., Hasanoğlu Özkan E., Arslan H.
Biotechnology and Applied Biochemistry, cilt.64, ss.745-753, 2017 (SCI-Expanded)
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Yayın Türü:
Makale / Tam Makale
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Cilt numarası:
64
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Basım Tarihi:
2017
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Doi Numarası:
10.1002/bab.1533
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Dergi Adı:
Biotechnology and Applied Biochemistry
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Derginin Tarandığı İndeksler:
Science Citation Index Expanded (SCI-EXPANDED), Scopus
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Sayfa Sayıları:
ss.745-753
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Anahtar Kelimeler:
biosensor, carbon paste, glucose oxidase, microsphere, polystyrene, Schiff base, AMPEROMETRIC DETECTION, COMPOSITE, NANOPARTICLES, NANOFIBER, SENSORS, FILM
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Gazi Üniversitesi Adresli:
Evet
Özet
In the present study, a novel biosensor that is sensitive to glucose was prepared using the microspheres modified with (4-formyl-3-methoxyphenoxymethyl)polystyrene (FMPS) with L-glycine. Polymeric microspheres having Schiff bases were prepared from FMPS using the glycine condensation method. Glucose oxidase enzyme was immobilized onto modified carbon paste electrode by cross-linking with glutaraldehyde. Oxidation of enzymatically produced H2O2 (+0.5 V vs. Ag/AgCl) was used for determination of glucose. Optimal temperature and pH were found as 50 ◦C and 8.0, respectively. The glucose biosensor showed a linear working range from 5.0 × 10−4 to 1.0 × 10−2 M, R2 = 0.999. Storage and operational stability of the biosensor were also investigated. The biosensor gave perfect reproducible results after 20 measurements with 3.3% relative standard deviation. It also had good storage stability.