This study compared the somatotype values of football players according to their playing positions. The study aimed to determine the physical profiles of players and to analyze the relationships between somatotypes and playing positions. Study participants were members of two teams in the Turkey Professional Football League, Genclerbirligi Sports Team (GB) (N = 24) and Genclerbirligi Oftas Sports Team (GBO) (N = 24). Anthropometric measurements of the players were performed according to techniques suggested by the Anthropometric Standardization Reference Manual (ASRM) and International Biological Program (IBP). In somatotype calculations, triceps, subscapular, supraspinale and calf skinfold thickness, humerus bicondylar, femur bicondylar, biceps circumference, calf circumference and body weight and height were used. Statistical analysis of the data was performed using the Graph Pad prism Version 5.00 for Windows (Graph Pad Software, San Diego California USA); somatotype calculations and analyses used the Somatotype 1.1 program and graphical representations of the results were produced. Analysis of non-parametric (two independent samples) Mann-Whitney U Test of the player data showed that there were no statistically significant differences between the two teams. The measurements indicated that, when all of the GB and GBO players were evaluated collectively, their average somatotypes were balanced mesomorph. The somatotypes of GBO goalkeepers were generally ectomorphic mesomorph; GB goalkeepers were balanced mesomorphic, although they were slightly endomorphic.