Quantitative determination of fexofenadine in human plasma by HPLC-MS

Isleyen E. A. O. , Oezden T., Oezilhan S., Toptan S.

CHROMATOGRAPHIA, vol.66, 2007 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 66
  • Publication Date: 2007
  • Doi Number: 10.1365/s10337-007-0267-x
  • Journal Indexes: Science Citation Index Expanded, Scopus


A simple, rapid, sensitive and selective LC-MS method was developed and validated for quantification of fexofenadine in human plasma. The LC-MS system was operated under the positive electrospray ionisation mode (ESI). After liquid-liquid extraction, fexofenadine analysis was performed on a C-18 column with a mobile phase of acetonitrile: 10 mM ammonium acetate: formic acid, 70:30:0.1 (v/v/v) at a flow rate of 1 mL min(-1) by using loratacline as internal standard. The lower limit of quantitation was 3 ng mL(-1) for fexofenadine. The assay precision ranged between 1.05 and 12.56% and accuracy ranged between 82.00 and 109.07%. The validated method was successfully used to analyze human plasma samples in bioequivalence studies.