Inhibitory Efficacy of Lactic Acid Bacteria Against Biofilm Production of Deletion Mutants of Salmonella Typhimurium 14028 Strain in terms of dam and seqA Genes


Göksel Ş.

III. INTERNATIONAL AGRICULTURAL, BIOLOGICAL & LIFE SCIENCE CONFERENCE, Edirne, Türkiye, 1 - 03 Eylül 2021, ss.230

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: Edirne
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.230
  • Gazi Üniversitesi Adresli: Evet

Özet

In this study, the effects of culture filtrates of lactic acid bacteria inhibit the growth of planktonic forms of S. Typhimurium 1408 wild-type strain on biofilm formation and eradication in mutants with deletion of a dam, and seqA genes of the said bacterium were investigated. As a result of the trials carried out on polystyrene microtitre plates, it was determined that 6 lactic acid bacteria (Lactobacillus plantarum, Lactobacillus sake, Pediococcus acidilactici, Bifidobacterium longum, Bifidobacterium bifidum, and Pediococcus pentosaceous) used in the trials completely inhibited biofilm formation in these mutants at optimum biofilm production times. In these experiments, culture filtrates were inoculated into biofilmproducing media together with the biofilm producer strain. In the eradication studies of biofilm structures, culture filtrates were added to the medium after the biofilm structures matured. In eradication studies carried out in this way, it was determined that the culture filtrates of lactic acid bacteria did not show any biofilm eradication efficiency.