The Use of Boron Compounds for Stabilization of Lipase from Pseudomonas aeruginosa ES3 for the Detergent Industry


SARAÇ N., UĞUR A. , BORAN R., ELGİN E. S.

JOURNAL OF SURFACTANTS AND DETERGENTS, vol.18, no.2, pp.275-285, 2015 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 2
  • Publication Date: 2015
  • Doi Number: 10.1007/s11743-014-1653-7
  • Title of Journal : JOURNAL OF SURFACTANTS AND DETERGENTS
  • Page Numbers: pp.275-285
  • Keywords: Pseudomonas aeruginosa, Lipase, Boron, Stabilization, Detergent, Antimicrobial, SOLVENT-TOLERANT LIPASE, ALKALINE LIPASE, MOLECULAR CHARACTERIZATION, PARTIAL-PURIFICATION, PROTEASE

Abstract

This study aimed to characterize a lipase that is highly active and stable under typical washing conditions for use as a detergent ingredient by investigating the effects of various boron compounds on lipase stabilization under different conditions. In addition, the antimicrobial activity of the boron compounds used in enzyme stabilization was examined in order to obtain an effective antimicrobial detergent. A lipase-producing bacterium was isolated from kitchen wastewater samples using Rhodamine-B Agar medium and identified as Pseudomonas aeruginosa based on 16S rDNA sequence analysis. The ES3 lipase obtained from P. aeruginosa was purified, and the purified enzyme was found to have a molecular mass of 40 kDa. The enzyme showed optimal activity at pH 9.0-10.0 and 40 A degrees C and remained stable in the presence of various metal ions, surfactants and oxidizing agents. Moreover, the pH stability and thermostability of the enzyme was improved by the addition of boron compounds, which, when used as stabilizers in the incubation media, also increased the stability of the enzyme towards commercial detergents. Furthermore, the enzyme displayed properties comparable with the commercial product Lipolase(A (R)), which has shown excellent stability towards various commercial detergents. Finally, boron compounds used to stabilize the lipase were found to possess antimicrobial properties, suggesting that detergents incorporating these compounds will also exhibit antimicrobial activity when washing clothes and dishes.