The effect of tryptophan administration on ileum contractility and oxidant status in mice


Ozer Ç., Gonul B., Ercan Z. S. , Take G., Erdogan D.

AMINO ACIDS, vol.32, no.3, pp.453-458, 2007 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 3
  • Publication Date: 2007
  • Doi Number: 10.1007/s00726-006-0339-5
  • Journal Name: AMINO ACIDS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.453-458
  • Keywords: tryptophan, serotonin, ileal contractility, oxidant status, immunohistochemistry, GASTROINTESTINAL MOTILITY, LIPID-PEROXIDATION, BRAIN-SEROTONIN, SMALL-INTESTINE, RATS, RECEPTORS, 5-HYDROXYTRYPTAMINE, LOCALIZATION, HYDROXYLASE, REPERFUSION
  • Gazi University Affiliated: Yes

Abstract

L-Tryptophan (TRP) is the precursor amino acid for the synthesis of serotonin (5-HT). 5-HT is effective both on the food intake and gastrointestinal system contractility. The aim of this study was to search the effects of systemic TRP treatment on 5-HT levels of ileum and searching the effect of ileal contractility and oxidant status. Swiss-albino mice were divided into two groups: 1. Control, 2. TRP-treated (100 mg/kg/24 h, i.p., for 7 days). Body weights were recorded at the beginning and at the end of experiments. Acetylcholine-induced contractile responses in the isolated ileum were recorded on polygraph. Ileal tissue malondialdehyde and glutathione levels determined by spectrophotometric and ileal tissue 5-HT levels were measured by immunohistochemical methods. TRP treatment decreased body weight and increased ileal contractile response. In the TRP-treated group, ileum malondialdehyde levels increased and glutathione levels decreased. Immunohistochemical detection showed that ileal 5-HT levels were increased by TRP treatment. There is a relationship between increased oxidative stress and increased contractility in the ileal tissue of the TRP-treated animals. These effects may be related to increased ileal 5-HT synthesis.