Akademik Veri Yönetim Sistemi
Veterinary Parasitology, cilt.343, 2026 (SCI-Expanded, Scopus)
The increasing resistance of gastrointestinal nematodes (GINs) to conventional anthelmintic drugs has intensified the search for alternatives. This study evaluated the anthelmintic efficacy of Avena sativa L. seed extracts using in vitro and in silico approaches against GINs of sheep. Aqueous (AS–H₂O) and n-hexane (AS–n-hexane) extracts prepared from A. sativa seeds were tested against GINs using egg hatch and larval motility inhibition assays. Both extracts demonstrated clear dose-dependent ovicidal activity, achieving 100 % egg hatch inhibition at concentrations ≥ 12.5 mg/mL, while lower concentrations (6.25–1.5625 mg/mL) produced partial but substantial inhibition. Nonlinear four-parameter logistic modelling yielded EC₅₀ values of 2.16 mg/mL for AS–H₂O and 3.14 mg/mL for AS–n-hexane, indicating greater ovicidal potency of the aqueous extract. The AS–H₂O extract exhibited superior anthelmintic activity, with a 24 h mortality rate of 81.33 % and an LC₅₀ value of 3.62 mg/mL, compared to 74.66 % and 6.24 mg/mL for the AS–n-hexane extract. LC–MS/MS analysis of AS–H₂O extract identified six phenolic compounds: fumaric acid, (−)-quinic acid, protocatechuic acid, protocatechuic aldehyde, naringenin, and apigenin. Molecular docking revealed strong binding affinities of these compounds—particularly naringenin (−8.4 kcal/mol)—to the colchicine-binding site of β-tubulin, a key antiparasitic target. These findings highlight the potential of A. sativa seed extracts, especially the aqueous form, as natural and sustainable anthelmintic agents for parasite control in livestock.