A novel Trans-Pt(II) complex bearing 2-acetoxymethylbenzimidazole as a non-leaving ligand (trans-[Pt(AMBi)(2)Cl-2]): Synthesis, antiproliferative activity, DNA interaction and molecular docking studies compared with its cis isomer (cis-[Pt(AMBi)(2)Cl-2])


EREN G., EMERCE E., AÇIK L., AYDIN B., Gumus F.

JOURNAL OF MOLECULAR STRUCTURE, cilt.1184, ss.512-518, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1184
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.molstruc.2019.02.066
  • Dergi Adı: JOURNAL OF MOLECULAR STRUCTURE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.512-518
  • Anahtar Kelimeler: Platinum complexes, Trans configuration, Benzimidazole, Docking, MTT, DNA binding, PLATINUM(II) COMPLEXES, CYTOTOXIC ACTIVITY, ANTICANCER ACTIVITY, BINDING, CISPLATIN, CHEMISTRY, CRYSTAL, ADDUCTS, GROWTH, PT(II)
  • Gazi Üniversitesi Adresli: Evet

Özet

Trans-[Pt(AMBi)(2)Cl-2 (1) in which AMBi is 2-acetoxymethylbenzimidazole, was synthesized and characterized by spectroscopic methods. Novel complex 1 was evaluated for its potential antiproliferative effect against three human cancer cell lines, including HeLa (human cervix cancer), MCF-7 (human breast cancer) and A549 (human non-small-cell lung cancer) in comparison with those of cis-[Pt(AMBi)(2)Cl-2 (2) and cisplatin by means of the MTT assay. Interaction of complexes 1, 2 and cisplatin with pBR322 plasmid DNA and their restriction endonuclease reactions by BamHI and HindIII enzymes were studied by agarose gel electrophoresis. Cytotoxicity tests showed that complex 1 exhibited similar in vitro cytotoxicity profile as compared with cisplatin whereas better than corresponding cis isomer against MCF-7 and A549 human cancer cell lines. The electrophoretic mobility shift assay showed that although no comigration of the Form I and II bands of plasmid DNA was observed for complex 1 at the concentrations tested, the binding of complex 1 was able to prevent partially BamHI digestion for concentrations ranging from 40 to 160 mu M while having no inhibitory effect on HindIII activity in the digestion studies with restriction enzymes. Molecular docking studies on DNA were carried out to explore the binding mode and the best orientation of the complexes 1 and 2 to DNA. (C) 2019 Elsevier B.V. All rights reserved.