An amperometric biosensor for choline determination prepared from choline oxidase immobilized in polypyrrole-polyvinylsulfonate film

Ozdemir, Merve; ARSLAN, FATMA; ARSLAN, HALİT

doi:10.3109/10731199.2011.646411

An amperometric biosensor for choline determination prepared from choline oxidase immobilized in polypyrrole-polyvinylsulfonate film

Atıf İçin Kopyala

Ozdemir M., ARSLAN F., ARSLAN H.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, cilt.40, sa.4, ss.280-284, 2012 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 40 Sayı: 4
Basım Tarihi: 2012
Doi Numarası: 10.3109/10731199.2011.646411
Dergi Adı: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.280-284
Gazi Üniversitesi Adresli: Evet

Özet

In this paper, a novel amperometric choline biosensor with immobilization of choline oxidase on electrochemically polymerized polypyrrole-polyvinylsulphonate (PPy-PVS) film has been accomplished via the entrapment technique. The effects of pH and temperature were investigated and optimum parameters were found to be 9.0 and 60 degrees C, respectively. There are two linear parts in the region between 1.0 x 10(-7) -1.0 x 10(-6) M (R-2 = 0.997) and 1.0 x 10(-5) -1.0 x 10(-3) M (R-2 = 0.986). The storage stability and operation stability of the enzyme electrode were also studied.