An amperometric biosensor for choline determination prepared from choline oxidase immobilized in polypyrrole-polyvinylsulfonate film


Ozdemir M., ARSLAN F. , ARSLAN H.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, cilt.40, sa.4, ss.280-284, 2012 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 40 Konu: 4
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3109/10731199.2011.646411
  • Dergi Adı: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Sayfa Sayıları: ss.280-284

Özet

In this paper, a novel amperometric choline biosensor with immobilization of choline oxidase on electrochemically polymerized polypyrrole-polyvinylsulphonate (PPy-PVS) film has been accomplished via the entrapment technique. The effects of pH and temperature were investigated and optimum parameters were found to be 9.0 and 60 degrees C, respectively. There are two linear parts in the region between 1.0 x 10(-7) -1.0 x 10(-6) M (R-2 = 0.997) and 1.0 x 10(-5) -1.0 x 10(-3) M (R-2 = 0.986). The storage stability and operation stability of the enzyme electrode were also studied.