QUANTITATIVE DETERMINATION OF PARACETAMOL, CAFFEINE AND CODEINE PHOSPHATE IN PHARMACEUTICAL DOSAGE FORMS BY USING CAPILLARY ELECTROPHORESIS METHOD


Cakir K., Erkmen C., USLU B., GÜNDEN GÖĞER N.

REVUE ROUMAINE DE CHIMIE, vol.64, no.9, pp.801-808, 2019 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 64 Issue: 9
  • Publication Date: 2019
  • Doi Number: 10.33224/rrch.2019.64.9.07
  • Title of Journal : REVUE ROUMAINE DE CHIMIE
  • Page Numbers: pp.801-808

Abstract

A new capillary electrophoresis method has been developed to analysis of paracetamol (PAR), caffeine (CAF) and codeine (COD) phosphate from pharmaceutical dosage forms. Good results were obtained by using a background electrolyte solution consist of 25 mM (pH 8.5) phosphate buffer solution containing 10% methanol. The separation was performed through an uncoated fused-silica capillaries (50 mu m internal diameter, 56 cm total length, 47.5 cm effective length) at 25 degrees C with the application of 10 seconds of hydrodynamic injection at 40 mbar pressure and a potential of 27 kV. Detection wavelength was chosen 210 nm. Under optimum conditions, the migration times were found 4.427 min for PAR, 3.952 min for CAF, and 3.253 min for COD-phosphate. Linearity ranges were determined 99.72 - 1994.40 mu g mL(-1) for PAR, 5.75 - 115.04 mu g mL(-1) for CAF and 1.84 - 36.80 mu g mL(-1) for COD-phosphate for the method. Limit of detections were found 0.724 mu g mL-1 for PAR, 0.533 mu g mL(-1) for CAF and 0.263 mu g mL(-1) for COD-phosphate in this study. Also, limit of quantitations were found 2.415 mu g mL(-1) for PAR, 1.776 mu g mL(-1) for CAF and 0.877 mu g mL(-1) for COD-phosphate. According to the obtained results, the method is sensitive, specific and repeatable to analysis of PAR, CAF and COD-phosphate.