In vitro anticholinesterase activity of various alkaloids


Orhan İ. , Naz Q., Kartal M., Tosun F., Sener B., Choudhary M. I.

Zeitschrift fur Naturforschung - Section C Journal of Biosciences, vol.62, pp.684-688, 2007 (Journal Indexed in SCI Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 62
  • Publication Date: 2007
  • Doi Number: 10.1515/znc-2007-9-1010
  • Title of Journal : Zeitschrift fur Naturforschung - Section C Journal of Biosciences
  • Page Numbers: pp.684-688

Abstract

In the current study, a number of alkaloids including retamine, cytisine, and sparteine (quinolizidine-type), yohimbine and vincamine (indole-type), scopolamine and atropine (tropane-type), colchicine (tropolone-type), allantoin (imidazolidine-type), trigonelline (pyridine-type) as well as octopamine, synephrine, and capsaicin (exocyclic amine-type) were tested in vitro for their inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) at 1 mg/ml concentration by the Ellman method using an ELISA microplate reader. Among the alkaloids tested, only capsaicin exerted a remarkable inhibitory effect towards both AChE and BChE [(62.7 ± 0.79)% and (75.3 ± 0.98)%, respectively]. While the rest of the alkaloids did not show any significant inhibition against AChE, three of the alkaloids, namely retamine, sparteine, and yohimbine, exerted a noteworthy anti-BChE effect as compared to galanthamine, the reference drug. © 2007 Verlag der Zeitschrift für Naturforschung.