Preparation of carbon paste electrode containing polyaniline-activated carbon composite for amperometric detection of phenol
Bulgarian Chemical Communications, cilt.50, sa.1, ss.16-20, 2018 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 50 Sayı: 1
- Basım Tarihi: 2018
- Dergi Adı: Bulgarian Chemical Communications
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.16-20
- Anahtar Kelimeler: Phenol, polyphenol oxidase, biosensor, polyaniline (pani), polyaniline activated carbon composite, carbon paste, POLYPHENOL OXIDASE, TYROSINASE BIOSENSOR, ENZYME ELECTRODE, WINES
- Gazi Üniversitesi Adresli: Evet
Özet
http://www.bcc.bas.bg/BCC_Volumes/Volume_50_Number_1_2018/BCC-50-1-2018-16-20-4743-Arslan.pdf© 2018 Bulgarian Academy of Sciences, Union of Chemists in Bulgaria.In this study, a novel carbon paste electrode was prepared using the salt form of polyaniline (pani)-activated carbon composite sensitive to phenol. Polyphenol oxidase enzyme was immobilized to the modified carbon paste electrode by cross-linking with glutaraldehyde. The amperometric determination is based on the electrochemical reduction of o-quinone generated in the enzymatic reaction of phenol at -0.15 V vs. Ag/AgCl. The effects of pH and temperature were investigated and optimum values were found to be 8.0 and 45 °C, respectively. The linear working range of the electrode was 1.0×10-6 - 5.0×10-5 M, R2 =0.9819. The storage stability and operation stability of the enzyme electrode were also studied.