Karyotype, C-banding and AgNORs of two endemic leuciscine fish, Pseudophoxinus crassus (Ladiges, 1960) and P. hittitorum Freyhof & Ozulug, 2010 (Teleostei, Cyprinidae)

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Unal S., GAFFAROĞLU M., Ayata M. K., Yuksel E.

COMPARATIVE CYTOGENETICS, cilt.8, sa.4, ss.249-257, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8 Sayı: 4
  • Basım Tarihi: 2014
  • Doi Numarası: 10.3897/compcytogen.v8i4.7623
  • Dergi Adı: COMPARATIVE CYTOGENETICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.249-257
  • Gazi Üniversitesi Adresli: Evet

Özet

The genus Pseudophoxinus Bleeker, 1860 is found in a wide range of habitats in central Anatolia, but it is not well known from a cytogenetic aspect. In this study the first karyotypic description of the spring minnows Pseudophoxinus crassus (Ladiges, 1960) and P. hittitorum Freyhof & Ozulug, 2010 by means of conventional methods (Giemsa staining, C-banding, silver nitrate impregnation (Ag-NORs)) was performed. Both species are endemic and have restricted distributions in Central Anatolia. P. crassus and P. hittitorum have the same diploid chromosome number, 2n = 50, patterns of distribution of constitutive heterochromatin (CH), and localization of nucleolus organizer regions (NORs), but differ in their karyotypic formulae (KFs). The C-banding technique revealed clear pericentromeric blocks of CH in many chromosomes; Ag-NORs treatment revealed consistent positive signals at the end of the short arms of a submetacentric chromosome pair, likely homologous in both species. The karyotypic differences found between these species can be used for their taxonomical study.