In this study, the capacity of 30 strains of lactobacilli to coaggregate with Candida albicans ATCC 10239, C. albicans AJD 180 and Candida krusei ATCC 6258 were studied in vitro. A marked coaggregation with C. albicans was observed for two strains of Lactobacillus crispatus, a strain of Lactobacillus cellobiosus and a strain of Lactobacillus salivarius. Coaggregation occurred at a pH range from 3 to 7, some strains showing optimal binding at high and other strains at low pH. Treatment of lactobacilli at 70 or 85 degrees C for 20 min or treatment of the bacteria with pepsin abolished their capacity of coaggregation. The results may be of importance when trying to establish probiotics for vaginal use.