Conclusion. The results suggest that vitamin A can prevent scar formation in the vocal fold after surgery. Objectives. This study aimed to evaluate the effects of topically applied vitamin A on healing after vocal fold trauma. Materials and methods. Vocal folds of 20 adult rabbits were traumatized unilaterally. Ten of them were treated with topical application of vitamin A and the others served as controls. All animals were sacrificed after 10 days. Vocal folds were resected for analysis by light microscopy. Results. The untreated vocal folds showed extensive deposition of collagen and fibroblast on light microscopy and vocal folds treated with vitamin A showed less deposition. There was a significant difference between the two groups according to the percentage of collagen and fibroblasts in the lamina propria (p0.01).