Nucleoside analogue electrochemical behaviour and in situ evaluation of DNA-clofarabine interaction

Şatana H. E., Pontinha A. D. R., Diculescu V. C., Oliveira-Brett A. M.

BIOELECTROCHEMISTRY, cilt.87, ss.3-8, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 87
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1016/j.bioelechem.2011.07.004
  • Dergi Adı: BIOELECTROCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.3-8
  • Anahtar Kelimeler: Clofarabine, DNA, Oxidation, Voltammetry, PHARMACOLOGY, PLASMA, PURINE
  • Gazi Üniversitesi Adresli: Evet

Özet

Nucleoside analogues have had a substantial impact on the treatment of cancer, especially haematological malignancies. The electrochemical behaviour of the nucleoside analogue clofarabine (CLF) was investigated at a glassy carbon electrode using cyclic, differential pulse and square wave voltammetry in different pH supporting electrolytes. The oxidation process of CLF is irreversible and pH-dependent with transfer of two protons and two electrons, following a diffusion controlled mechanism. The oxidation mechanism of CLF involves deprotonation and leads to the formation of a hydroxylated species that undergoes reversible redox reactions. The interaction of DNA and the antileukemia drug CLF was investigated using a dsDNA-electrochemical biosensor in incubated solutions by differential pulse voltammetry. The CLF-DNA interaction leads to changes in the DNA morphological structure, confirmed using the purinic homo-polynucleotide single stranded sequences of guanosine and adenosine, poly[G] and poly[A]-electrochemical biosensors. (c) 2011 Elsevier B.V. All rights reserved.