Introduction: Occupational injuries among healthcare workers adversely affect the quality of healthcare services by undermining their physical and mental well-being. This study evaluates the frequency, characteristics, and influencing factors of occupational injuries among non-physician healthcare workers in a university hospital. Methods: This cross-sectional study examines occupational injuries reported between 2020 and 2023 at a university hospital in Turkey. Variables included sociodemographic characteristics, occupation, department, working hours, cause and type of injury, time of injury, affected body part, use of personal protective equipment (PPE), medical intervention, and incapacity for work. Results: A total of 694 occupational injuries were reported at Gazi University Hospital between 2020 and 2023, with the fewest cases occurring in 2021. Among the injured workers, 58.8% were female, 89.2% were aged between 20 and 49 years, 30.1% did not use PPE, 76.4% received medical intervention, and 11.1% experienced incapacity for work. Cleaning workers (33.6%) and nurses (32.1%) accounted for the highest proportion of injuries. Sharps injuries were the most common type (48.8%), while injuries to the hands, fingers, and wrists comprised 53.3% of cases. The highest frequency of injuries occurred between 11:00 and 11:59 am. Sharps injuries were significantly associated with gender, age, educational background, occupation, working hours, and injury time. In contrast, cases of workplace violence were significantly associated with gender, occupation, and working hours. Conclusions: Sharps injuries and violence represent critical occupational hazards. Preventive strategies should be tailored to healthcare workers’ occupational roles and educational levels. Effective surveillance systems and reporting mechanisms should be implemented to promote workplace safety and reduce the risk of injuries.