Karyotypic characters, mitotic metaphase chromosomes, monoploid ideograms and karyograms of Dianthus L. section Fimbriati Boiss., D. tabrisianus Bien. ex Boiss., D. sessiliflorus Boiss., D. stramineus Boiss. & Heldr., and D. erythrocoleus Boiss. were investigated for the first time. Analysis of somatic metaphases showed that the chromosome numbers and the karyotype formula of these taxa were 2n = 30 = 22m + 6sm + 2st for D. tabrisianus, 2n = 30 = 14m + 8sm + 8st for D. sessiliflorus, 2n = 30 = 20m + 4sm + 6st for D. stramineus and 2n = 30 = 18m + 8sm + 4st D. erythrocoleus. No satellites were observed in the karyotypes of these species. The interchromosomal and intrachromosomal karyotype asymmetries were estimated with coefficient of variation of chromosome length (CVCL) and mean centromeric asymmetry (M-CA).