Gossypol induced apoptosis in the human promyelocytic leukemia cell line HL 60


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Balci A., Sahin F., Ekmekci A.

TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, vol.189, no.1, pp.51-57, 1999 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 189 Issue: 1
  • Publication Date: 1999
  • Doi Number: 10.1620/tjem.189.51
  • Title of Journal : TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE
  • Page Numbers: pp.51-57

Abstract

In human promyelocytic leukemia cell line HL60, apoptosis was induced by treatment with gossypol that is an inhibitor of protein kinase C. Gossypol acetic acid mas added to HL 60 cells at 50, 100, 180 and 200 mu M concentrations for six hours. Morphological features of apoptosis as well as internucleosomal DNA fragmentation mere evaluated by light microscope, agarose gel electrophoresis and spectrofluorometric quantitation. Our results indicated that with the effective concentrations of gossypol (50 and 100 mu M), apoptosis was induced in HL 60 cells.