This study was planned for the purpose of investigating the effect of hand preference on hand anthropometric measurements, with the participation of 393 healthy university students. The hand preference direction of the subjects was established according to the Edinburgh Inventory, and five hand preference determination groups were constituted after calculation of the laterality score. During performance of the hand anthropometric measurements, values for both hands were calculated, and seven parameters were evaluated for each hand. Hand width and shape index were found to be high for the right hand, but the palmar length/width ratio was found to be high for the left hand, for strong and weak right handers, respectively. No significant statistical values were obtained among ambidextrous subjects, although their parameters resembled these groups' values. Additionally, in the left-handed group, reverse direction asymmetric values were established, but significant statistical values were not obtained.