Akademik Veri Yönetim Sistemi
3rd International Gazi Pharma Symposium Series , Ankara, Türkiye, 8 - 10 Eylül 2021, ss.65
The fecal microbiota may be influenced by many factors including diet. High-fructose consumption might be a risk factor for several metabolic diseases. Kefir, which is known as a diary probiotic mixture, has several health improving effects, but its mechanism of action remains mostly unclear. This study aimed to evaluate the effects of fructose and kefir onthe combination of fecal microbiota in rats. Fructose was given to the male Wistar rats as a20% solution in drinking water for 15 weeks. Kefir supplementation (1ml/100 g body weight)was performed by gastric gavage once a day during final 6 weeks. Bacterial content of microbiota was determined with 16S rRNA sequencing and evaluated at the genus level. Dietary high-fructose significantly increased the relative abundance of Lactobacillus genus,but interestingly decreased the diversity of the Lactobacillus. Kefir treatment did not affect the Lactobacillus abundance, however markedly raised the variety of the Lactobacillus. Helicobacteracea abundance was also increased in fructose-fed rat, which responded tokefir supplementation with a significant decrease. Fructose rich diet also markedly increased the dybiosis apparent at genus level, including Blautia, Alistipes, Oscillibacterabundance, but kefir treatment decreased relative abundance of Blautia, Alistipes, but not Oscillibacter. In conclusion, dietary high-fructose may cause to unfavourable changes in the fecal microbiota; however kefir treatment exerted to improve the diversity of the microbiota. These findings could provide important information for understanding in dietary factor-induced alteration in composition of microbiota.