A new and simple differential pulse polarographic method for the analysis of wine has been established. With this method, it was possible to determine simultaneously six trace elements in wine. There was no need for time consuming extraction and separation procedures with danger of contamination. The polarogram of wet digested wine was taken initially in pH 2 acetate buffer and Pb, Cd, and Zn were determined by standard additions. Ethylene diamine tetraacetic acid (EDTA) was added and pH was increased to six by addition of NaOH. Fe and Cu were determined subsequently. The ammonia buffer, pH 9.5, was identified as the best medium for separation and determination of Ni and Zn. The quantities of trace elements were found as Cu 290 +/- A 20 mu g L(-1), Fe 8960 +/- A 50 mu g L(-1), Pb 148 +/- A 17 mu g L(-1), Cd 16 +/- A 8 mu g L(-1), Zn 460 +/- A 25 mu g L(-1), and Ni 78 +/- A 17 mu g L(-1).