The purpose of this study is to examine the acceptance of technology among Turkish pre-service teachers using the TAM. This study was carried out with Turkish university students in the fall semester of 2009. Samples of pre-service teachers from two university departments, Science Education and Mathematics Education, were selected. A total of 773 pre-service teachers volunteered in this study. The self-report questionnaire in Teo (2009) was used to measure the seven constructs in this study. Analysis of covariance (ANCOVA) and Analysis of Variance (ANOVA) with age as covariate, dimensions as dependent variables and demographic variables (gender, residence, grade and owning of computers) as independent categorical variables were conducted. The results of this study demonstrated that daily computer usage and computer ownership have direct effect on technology acceptance of pre-service teachers. The results of this study suggest that pre-service teachers need to involve more in using computer in their class activities to develop the perception of technology use in the class when they teach. We have discussed this result according to older literature and have made some suggestion for future researchers.