Herein a new species, Alyssum amasianum, is described and illustrated from North Anatolia. The new species is very similar to A. hirsutum subsp. caespitosum but differs in the shape and indumentum of leaves, trichome type of the fruits, and margin of the seeds. The ecology, biogeography, and conservation status of the new species are discussed. The leaf, fruit, and seed surface micromorphology of A. amasianum, A. hirsutum subsp. caespitosum, A. hirsutum subsp. hirsutum, A. strigosum subsp. strigosum, and A. xanthocarpum were also examined by scanning electron microscope.