Akademik Veri Yönetim Sistemi
8th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2025) , Ankara, Türkiye, 17 - 19 Aralık 2025, ss.159, (Özet Bildiri)
Abstract Borax decahydrate (disodium tetraborate decahydrate) is one of the tincal derivatives and is widely used as an additive in many industrial sectors, such as agriculture, detergents, glass, ceramics, and cosmetic products. According to previous studies, boric acid and its derivatives have shown important biological effects such as potential antioxidant, anti-inflammatory and anticancer. However, studies on human lymphocytes regarding genotoxic potential are limited. The aim of this study is to evaluate the potential genotoxic effect of borax decahydrate using the micronucleus assay in human peripheral blood lymphocytes in vitro. This research was approved by the Gazi University Faculty of Medicine Ethics Committee (01.08.2025/137). Lymphocyte cultures were treated with five different concentrations of borax decahydrate (2.5, 5, 10, 20, and 40 µg/mL) for 48 hours. A negative (distilled water) and a positive control (Mitomycin-C, 0.20 μg/mL) were conducted in culture. According to the results, none of the studied concentrations of borax decahydrate caused a significant increase in the frequency of MN between 2.5 μg/mL and 40 μg/mL concentrations compared to the control. Additionally, borax decahydrate did not affect nuclear division index (NDI) at any treatment. These findings indicate that borax decahydrate did not exhibit genotoxic or cytotoxic effects in human peripheral lymphocytes under the tested concentrations in in vitro conditions. Keywords: Borax decahydrate, micronucleus assay, nuclear division index, human peripheral lymphocytes. *This study is supported by the Gazi University Research Fund under project number FYL-2025-10447