Relationships between seminal plasma composition and sperm quality parameters of the Salmo trutta macrostigma (Dumeril, 1858) semen: with emphasis on sperm motility


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Bozkurt Y., Ogretmen F., Kokcu O., Ercin U.

CZECH JOURNAL OF ANIMAL SCIENCE, vol.56, no.8, pp.355-364, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 56 Issue: 8
  • Publication Date: 2011
  • Doi Number: 10.17221/2394-cjas
  • Journal Name: CZECH JOURNAL OF ANIMAL SCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.355-364
  • Keywords: spermatological parameters, spermatozoa, seminal fluid, Salmo trutta macrostigma, CARP CYPRINUS-CARPIO, RAINBOW-TROUT, ONCORHYNCHUS-MYKISS, SPERMATOLOGICAL PARAMETERS, SPAWNING SEASON, PHYSICAL CHARACTERISTICS, MILT PRODUCTION, FRESH-WATER, SPERMATOZOA, FISH
  • Gazi University Affiliated: Yes

Abstract

The mineral and organic composition of seminal plasma, physical spermatological parameters and their physiological relationships were investigated in Salmo trutta macrostigma. The seminal plasma contained 121.0 +/- 0.37mM/lI (Na+), 8.18 +/- 0.03mM/l (K), 7.23 +/- 0.03 mg/dl (Ca2+), 3.19 +/- 0.02 mEq/l (Mg++), 0.48 +/- 0.02 g/dl total protein, 6.07 +/- 0.06 mg/dl cholesterol, 6.24 +/- 0.08 mg/dl triglyceride and 9.97 +/- 0.39 mg/dl urea. The following physical spermatological parameters were found out: sperm volume 13.93 +/- 0.84 ml, sperm motility 80.37 +/- 2.36%, movement duration 81.47 +/- 4.21 s, density 6.02 +/- 0.46 x 10(9)/ml, total density 8.85 +/- 6.12 x 10(9) and pH 7.53 +/- 0.20. Significant positive relationships were determined between motility duration and motility (r = 0.83, P < 0.01) and also between spermatocrit and motility (r = 0.536, P < 0.05). Sperm volume and total density negatively correlated with motility (r = -0.191, P > 0.05 and r = -0.087, P > 0.05, respectively). The Na+, K+ and Cl- ions correlated negatively with motility (r = -0.267, P > 0.05, r = -0.152, P > 0.05 and r = -0.461, P > 0.05, respectively). On the other hand, the Ca2+ and Mg2+ ions correlated positively with motility (r = 0.114, P > 0.05 and r = 0.040, P > 0.05, respectively). A significant negative relationship was found between motility and urea (r = -0.515, P < 0.05). These parameters should be considered when developing procedures for short-term storage or cryopreservation of the Salmo trutta macrostigma sperm.