© 2018 Novin Medical Radiation Institute. All Rights Reserved.Background: With the development of technology, human beings are increasingly under the exposure of Radio Frequency Radiation (RFR) (due to wireless technologies, mobile phones, base stations etc). The aim was to investigate the possible effects of Radio Frequency Radiation (RFR) on blood, aorta and heart tissue in hypertensive and non-hypertensive rats with various parameters. Materials and Methods: Male Wistar Albino rats were divided into 4 groups: 1.Control (C), 2.Hypertension (H), 3.RFR, 4.RFR+Hypertension (RFR+H). 60 mg/kg L-Nitroarginine Methyl Ester (L-NAME) was administered for 1 month by oral gavage to induce hypertension. The rats were exposed to 2100 MHz RFR for 60 minutes/day, 5 days/ week for 8 weeks. Blood hematocrit, blood and plasma viscosity, malondialdehyde (MDA), total nitric oxide (NOX), and Total sulphydril groups (RSH) / glutathione (GSH) levels in plasma and heart tissue were determined. Histological examination of the aortic wall and left ventricular muscle was performed. Results: RFR exposure resulted in a marked increase in cardiac and plasma MDA and NOX levels and a decrease in RSH/GSH levels (p<0.05). Blood viscosity increased in the hypertensive group with RFR exposure (p<0.05). There was an increase in left ventricular weight and number of muscle cells in hypertensive groups (p<0.05). Conclusion: According Our study suggests that exposure to RFR causes more negative effects on hypertension in terms of oxidative stress and antioxidants in the heart and plasma. Also, RFR exposure showed more prominent left ventricular fibrosis and aortic degenerative effects in hypertensive groups.