In vitro genotoxicity assessment of gadobutrol by comet assay

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Akbaş E., Ünal F., Yüzbaşıoğlu D.

5th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2022), Ankara, Turkey, 23 - 25 November 2022, pp.245

  • Publication Type: Conference Paper / Summary Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.245
  • Gazi University Affiliated: Yes


Magnetic  resonance  imaging  (MRI)  is  a  high  resolution  imaging  tool  that  enables  the  examination  of  anatomic, physiologic,  biochemical,  and  molecular  details  of  lesions  in  the  body.  In  40%  of  all  imaging,  contrast  agents  are administered to patients to better distinguish the tissues. Gadolinium-based contrast agents (GBCAs) have been widely used in clinical MR imaging studies for over three decades. Since gadolinium is toxic alone, it is bonded a chelating ligand  to  form  GBCAs.  Gadobutrol  is  one  of  the  most  commonly  used  GBCA  during  MRI,  that  has  non-ionic  and macrocyclic  chemical  structure.  According  to  the  recent  studies,  there  are  findings  showing  that  GBCAs  lead  to gadolinium retention in the body resulting in some toxic effects. Evaluations revealed both  positive and negative results in terms of genotoxicity as well. Therefore, the aim of this study was to assess the genotoxic potential of gadobutrol on human  peripheral  lymphocytes  by  comet  assay.  For  this  purpose,  the  isolated  lymphocytes  were  treated  with  7,000, 14,000, 28,000, 56,000, and 112,000 μg/mL concentrations of gadobutrol and incubated for 1 h at 37°C. There were also negative  (distilled  water)  and  positive  (Hydrogen  peroxide)  controls  in  the  experiments.  It  was  observed  that  all  the concentrations  of  gadobutrol  (except  14,000  μg/mL)  increased  tail  intensity  and  tail  moment  but  only  the  highest concentration  (112,000  μg/mL)  was  significant  in  tail  intensity. Increased  in  tail  intensity  and  tail  moment  might  be occurred because of the damage like oxidation resulted from free radical formation. Thus, these results can be interpreted like gadobutrol had a weak DNA damaging effect on isolated lymphocytes. Nonetheless, further investigations should be performed for better identification of genotoxic end points of gadobutrol under other in vitro and in vivo conditions.