alpha-Amylase and alpha-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa roots


KARAKAYA S., ÖZBEK H., GÖZCÜ S., GÜVENALP Z., YUCA H., DUMAN H., ...More

BANGLADESH JOURNAL OF PHARMACOLOGY, vol.13, no.1, pp.35-40, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 13 Issue: 1
  • Publication Date: 2018
  • Doi Number: 10.3329/bjp.v13i1.33668
  • Journal Name: BANGLADESH JOURNAL OF PHARMACOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.35-40
  • Gazi University Affiliated: Yes

Abstract

Eleven coumarins named osthole (1), imperatorin (2), bergapten (3), prants-chimgin (4), grandivitinol (5), suberosin (6), xanthotoxin (7), felamidin (8), marmesin (9), umbelliferone (10), ulopterol (11), and a sterol mixture consisted of stigmasterol (12), beta-sitosterol (13) were isolated from the roots of Ferulago blancheana, F. pachyloba and F. trachycarpa through in vitro bioassay-guided fractionation processes. The extracts and bioactive compounds were evaluated for their alpha-amylase and alpha-glucosidase activities. Among the tested compounds, felamidin and suberosin showed significant alpha-glucosidase inhibitory activity with IC50 values of 0.4 and 0.9 mg/mL, respectively, when compared to the reference standard acarbose (IC50 = 4.9 mg/mL). Grandivitinol (IC50 = 20.0 mg/mL) had the lowest inhibitory effect. On the other hand, none of the tested extracts were found to be active on alpha-amylase inhibition. This is the first report on isolation, characterization of the bioactive compounds and evaluation the alpha-amylase and alpha-glucosidase inhibitory activities of these species.