Development of an electrochemical DNA biosensor for detection of specific Mycobacterium tuberculosis sequence based on poly(L-glutamic acid) modified electrode

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Yesil M., DÖNMEZ S., ARSLAN F.

JOURNAL OF CHEMICAL SCIENCES, cilt.128, sa.11, ss.1823-1829, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 128 Sayı: 11
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1007/s12039-016-1159-0
  • Dergi Adı: JOURNAL OF CHEMICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1823-1829
  • Anahtar Kelimeler: Poly(L-glutamic acid), DNA sensor, Mycobacteriumtuberculosis, CARBON-PASTE ELECTRODE, DOPAMINE, HYBRIDIZATION, OXIDATION
  • Gazi Üniversitesi Adresli: Evet

Özet

An electrochemical DNA biosensor was developed by avidin-biotin interaction of a biotinylated probe and avidin-attached, poly(L-glutamic) acid coated pencil graphite electrode (PGA/PGE) for detection of specific Mycobacterium tuberculosis DNA sequence. The discrimination of fully complementary hybridization and mismatch hybridization was carried out by electrochemical reduction current of Meldola's Blue (MDB) in square wave voltammetry (SWV). The calibration graph of the DNA biosensor was linear between 1.5-12.5 nM and the detection limit was calculated as 1.3 nM. The proposed biosensor successfully discriminated short and long oligonucleotides related to DNA sequence of Mycobacterium tuberculosis in optimal condition.