Protective effect of alpha-lipoic acid against ischemia-reperfusion injury in retina


Yildirim Z., Emmez H., Kale A.

Oxidation Communications, vol.37, no.2, pp.555-562, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 2
  • Publication Date: 2014
  • Journal Name: Oxidation Communications
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.555-562
  • Keywords: LA, Oxidative stress, Spinal cord injury, TAS, TNF-α, Vitreous
  • Gazi University Affiliated: Yes

Abstract

Radical oxygen species produced after injury counteracts antioxidant activity and frequently causes severe oxidative stress for the tissues. Alpha-lipoic acid (LA) is a powerful antioxidant. The aim of the study was to investigate the effects of LA supplementation on the levels of tumor necrosis factor-a (TNF-a), malondialdehyde (MDA), and total antioxidant status (TAS) in eye after spinal cord trauma. Twenty-four adults, male, New Zealand rabbits were divided into ischemia (n=8), control (n=8), and treatment groups (n=8). The abdominal aorta was clamped for 30 min by an aneurysm clip, approximately 1 cm below the renal artery and 1 cm above the iliac bifurcation in ischemia and treatment groups. In the control group, only laparotomy was performed. 25 cm3 of saline in control group and 100 mg/kg LA were administered intraperitoneally in the treatment group after closure of the incision. The animals were killed 48 h after the operation. Vitreous samples were collected for analysis. Vitreous levels of TNF-α, MDA, and TAS were analysed according to markers of oxidative stress and inflammation. The vitreous MDA and TNF-a levels were significantly higher in the ischemia group when compared to the control group (p<0.05). The vitreous MDA and TNF-α levels were significantly lower in the LA group when compared to the ischemia group (p<0.05). There was no significant difference between the vitreous TAS levels of the groups (p>0.05). Although further studies considering different dose regimens and time intervals are required, the results of the our study prove that LA acid has favourable effects on experimental spinal cord ischemia-reperfusion injury.