This paper describes the synthesis and characterization of a novel monomer (6,9-bis(4-hexylthiophen-2-yl)-1-1H-indeno [2,1-b]quinoxalin-11-one (M1)). M1 monomer was electrochemically polymerized on the electrode surface and used as a sensing platform for the immobilization of laccase. Moreover, electrochemical and optical properties of the corresponding polymer were performed in detail. To the best of our knowledge, electrochemical and biosensor performance of the polymer were investigated for the first time. The PM1/Laccase were used in the fabrication of a new biosensor for determination of catechol in water samples by ampemmetric methods. Under optimized conditions, the calibration curve showed a linear range of 0.005-0.175 mM, with a limit of detection of 9.86 mu M and high sensitivity of 153.6 mu A/mMcm(2). The biosensor exhibited good stability, selectivity and high sensitivity toward catechol.