Siderophore production, serum resistance, hemolytic activity and extended-spectrum beta-lactamase-producing Klebsiella species isolated from milk and milk products

Gundogan N., Yakar U. A.

JOURNAL OF FOOD SAFETY, vol.27, no.3, pp.251-264, 2007 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 3
  • Publication Date: 2007
  • Doi Number: 10.1111/j.1745-4565.2007.00077.x
  • Title of Journal : JOURNAL OF FOOD SAFETY
  • Page Numbers: pp.251-264


This study aimed at the isolation and identification of Klebsiella spp. from dairy product to establish their public health significance by determining their virulence factors, antibiotic resistance and extended-spectrum P-lactamase (ESBL). Klebsiella pneumoniae, Klebsiella oxytoca and Klebsiella rhinoscleromatis were identified in 25 (58%), 11 (26%) and 7 (16%) isolates, respectively. A high prevalence of Klebsiella isolates had virulence factors such as siderophore production (63%), serum resistance (32.5%) and hemolytic activity (58%). ESBL-producing Klebsiella spp. was detected in 35% of the isolates. Resistance to the antimicrobial agents tested was found to be much higher in the ESBL-producing Klebsiella spp. than in non-ESBL-producing isolates. All ESBL-producing Klebsiella spp. showed high-level resistance to cephalosporins and monobactams. The majority of the serum resistant, siderophore, hemolysin and ESBL producers were K. pneumoniae.